11th National Conference on Laboratory Aspects of Tuberculosis Presentations

April 23-24, 2019 | Atlanta, GA

The conference schedule for the 11th National Conference on Laboratory Aspects of Tuberculosis is listed below. Presentations with links have been authorized to be posted on this website, but duplication is not authorized without the express written permission of the authors. If permission is granted, please cite both the author and “11th National Conference on Laboratory Aspects of Tuberculosis."

Tuesday, April 23, 2019

Opening Session and Keynote

• Welcome to the 11th National Conference on Laboratory Aspects of Tuberculosis
  Angie Schooley, Michigan Department of Health and Human Services

• Keynote Address - The Journey to a Whole Genome Sequencing Diagnostic Service for Mycobacteria spp.
  Derrick Crook, PhD, Public Health England

A Time and A Place: The Role of Next Generation Sequencing

Moderator: Angela Starks, PhD, US Centers for Disease Control and Prevention

• National Overview of Next Generation Sequencing Approaches for Tuberculosis
  Kelly Wroblewski, MPH, APHL

• One Stop Shop: New York State’s Approach to TB Diagnosis
  Joseph Shea, MS, New York State Department of Health, Wadsworth Center

• Targeted NGS for TB Drug Resistance Detection
  Linlin Li, PhD, California Department of Public Health, Microbial Diseases Laboratory

• “Where Do We Go From Here?” Panel and Group Discussion
  James E. Posey, PhD, US Centers for Disease Control and Prevention
  Edward P. Desmond, PhD, California Department of Public Health, Microbial Diseases Laboratory
  Vincent E. Escuyer, PhD, New York State Department of Health, Wadsworth Center

TB Case Studies: Lessons from the Field

Moderator: Monica Youngblood, MPH, US Centers for Disease Control and Prevention

• The Missing IS6110 Gene
  Drew Francis, ASCP(M), Arizona State Public Health Laboratory

• Communication Works!
  Robin Connelly, MMSc., M(ASCP), Georgia Public Health Laboratory
  

Back to the Basics — Guidelines, Algorithms, Reports, Oh My!

Moderator: Marie-Claire Rowlinson, PhD D(ABMM), Florida Bureau of Public Health Laboratories - Jacksonville

• Cliff Notes of Recently Published Recommendations and Guidelines
  Max Salfinger, MD, University of South Florida, College of Public Health

• MALDI-‘Tough’: Implementation and Lessons Learned from a High Volume Mycobacteriology Laboratory
  Frances Jamieson, MD, FRCPC, Public Health Ontario

• TB Laboratory Reports Panel Discussion: "Is It All Greek to You?"
  Jessica Gentry, Indiana State Department of Health
  Barbara Seaworth, MD, Texas Center for Infectious Disease
  Kimberly Townsend MPA, BSN, RN, Montgomery County Department of Health and Human Services
  Paula M. Snippes Vagnone, MT(ASCP), Minnesota Department of Health

Collecting Wisdom and Sharing Knowledge: Enhancing Multi-Generational Workforces

Co-Moderator: Yvette Vergnetti, Alaska State Public Health Laboratory
Co-Moderator: Angie Schooley, Michigan Department of Health and Human Services

• Addressing the Multigenerational TB Workforce
  Catherine Stakenas, MA, American Society for Clinical Pathology (ASCP)

• APHL Knowledge Retention Toolkit
  Lorelei Kurimski, MS, APHL
  Jaye Boman, MT (AMT), State Hygienic Laboratory at the University of Iowa

Wednesday, April 24, 2019

Generations — Working Together for TB Elimination (Roundtable)

Co-Moderator: Yvette Vergnetti, Alaska State Public Health Laboratory
Co-Moderator: Anne M Gaynor, PhD, APHL

Drug Susceptibility Testing — MICs, Challenges, and Results Interpretations

Moderator: Vincent E. Escuyer, PhD, New York State Department of Health, Wadsworth Center

• Discordant Results
  Beverly Metchock, DrPH, D(ABMM), US Centers for Disease Control and Prevention

• Are We Ready to Report MIC Results?
  Daniela Cirillo, PhD, San Raffaele Scientific Institute, Milan, Italy

• MIC Clinical Perspective
  Megan Ninneman, PA, Jackson Memorial Hospital, Miami, Florida

Data Driven Science in the TB Laboratory

Moderator: Stephanie Johnston, MS, US Centers for Disease Control and Prevention

• False Positive Investigation Toolkit
  Robert Domaoal, PhD, US Centers for Disease Control and Prevention

• Tuberculosis Laboratory Aggregate Report, 5th Edition — 2014–2017
  Monica Youngblood, MPH, MT (ASCP), US Centers for Disease Control and Prevention

• Assessing Laboratory Specific Data
  Cortney Stafford, MPH, US Centers for Disease Control and Prevention

• Panel Discussion:

• Jane Voyles, BS, Arkansas Public Health Laboratory

• Jasmine Guillet, MPH, BS, Massachusetts State Public Health Laboratory

• Tunisia King, BS, New Jersey Public Health and Environmental Laboratories

• Dorothy Baynham, BS, Tennessee Department of Health, Laboratory Services

• Denise Dunbar, BA, Texas Department of State Health Services

The Evolution of TB Research

Moderator: Tracy Dalton, PhD, US Centers for Disease Control and Prevention

• Host-Directed Approaches to TB Therapy
  Suraj Sable, PhD, DVM, US Centers for Disease Control and Prevention

• Novel TB Diagnostics
  Patricia Hall, PhD, MS, US Centers for Disease Control and Prevention

• An Overview of the TB Trials Consortium
  Anne E. Purfield, PhD, US Centers for Disease Control and Prevention

TB Case Studies: Lessons from the Field

Moderator: Cortney Stafford, MPH, MT (ASCP), Centers for Disease Control and Prevention

• Cross-Contamination — Beyond Laboratory Testing
  Jasmine Guillet, MPH, BS, Massachusetts Department of Public Health

• Saturday Night Fever
  Shaka Brown, Capital Congress

Awards and Closing Remarks

• Angie Schooley, BS, Michigan Department of Health and Human Services

• On the Front Lines of TB Testing Award: Hector Rivas, MPH, Los Angeles County Public Health Laboratory

• TB Lifetime Achievement Awards:

• Grace Lin, California Department of Public Health

• David Warshauer, PhD, Wisconsin State Laboratory of Hygiene
  

• Beverly Metchock, DrPH, US Centers for Disease Control and Prevention

Posters (List of all Abstracts)

1. An Evaluation of Testing Activities Undertaken by the National Public Health Laboratory Drug Susceptibility Testing (DST) Reference Center, 2016-2018
   N. Ancona¹, S. Yu², G. Lin², E. Desmond², T. Dalton³, S. Johnston³, A. Starks³, K. Wroblewski¹, A. Gaynor¹
   1) Association of Public Health Laboratories; 2) Microbial Disease Laboratory, California Department of Public Health; 3) US Centers for Disease Control and Prevention, Division of Tuberculosis Elimination, Laboratory Branch

2. Evaluation of Direct versus Concentrated NAAT on Respiratory Specimens for Rapid Diagnosis of Pulmonary Tuberculosis in a Local Jurisdiction
   M.H. Zhowandai¹, M. Ghajar¹, S. Prabhu¹, J. Low¹, C. Bittencourt², D. Constable², M. Crumpler¹, L. Thrupp²
   1) Orange County Health Care Agency, Santa Ana, CA; 2) University of California Irvine Health, Orange, California

3. Fast and Easy Extraction for Both TB Genotyping and WGS
   R. Kramer, K. Wells, L. Vibber, D. Gard, A. Schooley and M. Soehnlen
   Michigan Department of Health and Human Services, Bureau of Laboratories, Lansing, MI

4. Rapid Mycobacterial Identification using Real Time PCR and MALDI-TOF Testing
   H. Seymour, J. Vanneste, S. Church, A. Schooley and M. Soehnlen
   Michigan Department of Health and Human Services, Bureau of Laboratories, Lansing, MI

5. Setting up First-line Drug Susceptibility Testing (DST) Directly from a Positive Mycobacteria Growth Indicator Tube (MGIT): The Catalyst for a Structured Workflow in the Virginia Division of Consolidated Laboratory Services (DCLS) Tuberculosis (TB) Laboratory
   K. Milloy, S. McReynolds, R. Mehr, T. Bateman, B. Gardner, S. Kelley and R. Oglesby
   Division of Consolidated Laboratory Services, Richmond, VA

6. Drug Susceptibility Testing of Mycobacterium tuberculosis Complex Isolates at the Florida State Public Health Laboratory: A Six-year Study
   C. Chiribau, C. Tanner and M-C. Rowlinson, Florida Bureau of Public Health Laboratories, Jacksonville, FL

7. Comparison of Magnetic Beads Bridging Flocculation Technique with Gene Xpert MTB/RIF Assay for Diagnosis of Tuberculous Meningitis (TBM) in High Endemic Low Resource Settings
   K. Sharma, M. Modi, A. Sharma, S. Singh and P. Ray, Post Graduate Institute of Medical Education and Research, Chandigarh, India

8. Pyrazinamide Susceptibility by BACTEC MGIT 960: Are the Discrepancies Always due to the Test Method?
   D. Kohlerschmidt, S. Wolfe, M. Isabelle, J. Shea, T.A. Halse, K. Musser, V.E. Escuyer
   Wadsworth Center, New York State Department of Health, Albany, NY

9. Evaluation of Vitek MS v3.0 MALDI-TOF for Identification of Mycobacterium tuberculosis and Common Non Tuberculous Mycobacteria (NTM) Isolates from Clinical Specimens
   K. Sharma , R. Panwar, S. Sethi, M. Modi, S. Singh, A. Sharma and P. Ray
   Post Graduate Institute of Medical Education and Research, Chandigarh, India

10. Reduction of Phenotypic Susceptibility Testing through Implementation of Universal Whole Genome Sequencing of Mycobacterium tuberculosis Complex Isolates in New York State
    J. Shea, T.A. Halse, D. Kohlerschmidt, P. Lapierre, V.E. Escuyer and K.A. Musser
    Wadsworth Center, New York State Department of Health, Albany, NY

11. Evaluation of rpoB Mutations in Mycobacterium tuberculosis and their Association with Rifampin Resistance during 2.5 years of Prospective Side-by-side Whole Genome Sequencing and Phenotypic Testing
    J. Shea¹, T.A. Halse¹, D. Kohlerschmidt¹, M. Isabelle¹, S. Wolfe², A. Fiero², J.L. Rakeman², J. Lemon², P. Lapierre¹, V.E. Escuyer¹, K.A. Musser¹
    1) Wadsworth Center, New York State Department of Health, Albany, NY; 2) New York City Department of Health and Mental Hygiene, New York, NY

12. TB Puerto Rico Response to Hurricane Maria
    B. Jones¹, K. Milloy², M.R. Connelly³, A.M. Gaynor⁴, B. Metchock⁵, K. Klein⁵, S. Johnston⁵, M. Youngblood⁵, C. Stafford⁵, M. Yakrus⁵, C. Chiribau1, M-C. Rowlinson¹
    1) Florida Bureau of Public Health Laboratories-Jacksonville; 2) Virginia Division of Consolidated Laboratory Services: 3) Georgia Public Health Laboratory: 4) Association of Public Health Laboratories, 5) Division of TB Elimination, Centers for Disease Control and Prevention

13. Public-private Partnership Collaboration for Rapid Molecular Tuberculosis Testing in Guam
    A.C. Whelen¹, T. Koyamatsu¹, A.M. Santos², M.J. Jacar³, C. Henson³
    1) Diagnostic Laboratory Services, Inc. (DLS), Aiea, HI; 2) Department of Public Health and Social Services (GDPHSS), Mangilao, GU, 3DLS Guam, Tamuning, GU

14. Multi Targeted Loop-Mediated Isothermal Amplification (LAMP) for Rapid Diagnosis of Gastrointestinal tuberculosis in 60 Minutes
    S. Singh, K. Sharma, M. Modi, A. Sharma and P. Ray, Post Graduate Institute of Medical Education and Research, Chandigarh, India

Contact

For more information contact, Anne Gaynor, PhD, 240.485.2739, anne.gaynor@aphl.org.