Page Content APHL, in collaboration with CDC, is committed to assisting all PulseNet participating laboratories (USA and International) in achieving the highest quality of data before sharing sequencing results with their respective countries and/or regions. Part of that commitment consists of the development and implementation of standardized laboratory and analysis protocols that enable the exchange and comparison of whole genome sequence data between laboratories. The PulseNet Standard Operating Procedures (SOPs) provided below were established specifically for the PulseNet USA laboratories, with the understanding that most of the PulseNet USA standards will apply, within varying degrees, to our PulseNet International partners. We encourage PulseNet International laboratories to adopt these standards whenever possible and to modify them in a manner consistent with their internal policies or guidelines established by their institution, state, or country.For questions concerning PulseNet International SOPs, please contact pulsenet@cdc.gov. PulseNet SOPs PNIQ01_WGS Certification PND05 Analyzing Sequences Using PulseNet 2.0 v4 PND06 Cluster Detection PND20 RefID Database Workflow PNL33 DNA Extraction and Quality PNL34 Nextera XT Library Prep PNL34.W1 Nextera XT Library Prep Workbook for MiSeq v7 PNL35 Illumina DNA Prep PNL35.W1 Illumnia DNA Prep Workbook PNL36 QiaSeq Library Prep PNL37 Kapa Hyperplus Library Prep PNL39 WGS on MiniSeq PNL40 WGS on an iSeq PNL41 Ion Genestudio S5 PNL42 NEBNext Ultra II FS Library Prep PNL43 Illumina PCR-Free PNL44 PulseNet Rapid Library Prep SOP PNQ07 Illumina MiSeq data QC PNQ08 WGS Certification PNQ08 WGS Certification v9 PNQ09 WGS Proficiency Testing PNQ09 WGS Proficiency Testing v9 Library Kit Comparison Paper PNL34.W1 Nextera XT Library Prep Workbook for MiSeq v6.1 PNL35.JA1 Illumina DNA Prep Quick Guide_v3 PNL35.W1 Illumina DNA Prep Workbook_v17 PNL35.W3 Illumina DNA Prep Checklist_v8 PNL35.W4 Illumina DNA Prep Index Tracking_v5 PNL36.W1- PN QIAseq FX Library Prep Workbook PNL36-1 Appendix PN QIAseq FX Indices Tracking Worksheet PNL37.W1- PN KAPA HyperPlus Library Prep Workbook PNL37-1 Appendix PN KAPA HyperPlus Indices Tracking Worksheet PNL38 WGS on MiSeq SOP PNL41.W1 Ion Torrent S5 Workbook PNL42.W1_NEBNext_Ultra_II_FS_Workbook_v1 PNL44.W1 PulseNet Rapid Prep Workbook_v1 PNL44.W2 PulseNet Rapid Library Prep Checklist_v1 PNID02 Terra phylogenetic analysis PNID01 Terra analysis workflow It looks like your browser does not have JavaScript enabled. Please turn on JavaScript and try again.