TitleNon-robotic 8 channel reagent dispenserNon-Robotic 96 channel pipettor
Photo

Picture courtesy of Minnesota Department of Health

Picture courtesy of New England Newborn Screening Program              
Mode Of OperationUses tubing to dispense reagents 8 channels at a time (multi-dispense) to a plateAspirates and dispense 96 wells simultaneously from reservoir to plate or from plate to plate 
DNA Extraction-Used for addition of wash buffer, however buffer should be removed by hand or by vacuum apparatus.

-Used for addition of elution solution.

-Used for addition of wash buffer, however buffer should be removed by hand or by vacuum apparatus.

-Used for addition of elution solution.

PCR Set-Up -Used for addition of master mix. Small volume of excess solution may be recovered from tubing and re-used.

-Cannot be used for addition of DNA.
-Used for addition of master mix, however this is often an expensive reagent and a large excess volume must be added to the instrument to fill the 96 well reservoirs.

-Used for addition of DNA to either 96 or 384 well plate.
On Card Assay-Used for addition of wash buffer. Plate must be removed from instrument for incubation. Buffer should be removed by hand or by vacuum apparatus.

-Used for addition of PCR Master Mix addition.
 -Used for wash buffer addition. Plate must be removed from instrument for incubation. To avoid removal of DBS punch, buffer should be removed by hand or by vacuum apparatus.

-Used for addition of mastermix, however this is often an expensive reagent and a large excess volume must be added to the instrument to fill the 96 well reservoirs.
Deck Size1 plate dispensed at a time

Can hold up to 3 plates/reservoirs at a time.

Plate TypeDispensing head can adjusted to 12-, 24-, 48-, 96- 384-well microplate  Head cannot be adjusted. Fits a 96- and 384- well microplate.
Dispensing Mechanism A cassette is used that houses single rubber tube on one side and 8 smaller tubes on the other. The single tube on one side goes into the reagent. The tube then splits into 8 smaller tubes to dispense to the plate. While cassettes may be re-used, it is recommended to have a designated cassette for each reagent.Instrument can be purchased with fixed probes or can be used with removable tips. It is recommended that removable tips be used for molecular assays. The technician must manually change tray of tips between each dispense.
Filling A 96 Well PlateDispenses 8 wells at a time to fill a 96 well plate. Simultaneously fills 96 well plate.