​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​HIV virusAPHL develops and promotes HIV testing best practices and guidelines for diagnostic laboratories nationwide and supports public health laboratories in the implementation of national recommendations through close collaboration with CDC's Division of HIV Prevention. The estimated number of new HIV infections continues to decline despite the total number of persons living with HIV in the US continuing to increase. Accurate and early diagnosis of new HIV infections is imperative to the goal of an HIV-free future and our public health laboratories are actively engaged in this process.

​Laboratory Testing Algorithm 

APHL continues to monitor the HIV diagnostic landscape to provide members the most useful information. Since the 2014 HIV Laboratory ​Testing Algorithm, several resources have been developed to assist laboratories with understanding, using and reporting results from the diagnostic algorithm.

The results of the diagnostic algorithm may be used to identify persons likely​ to benefit from treatment, to reassure persons who are uninfected and for reporting evidence of HIV infection to public health authorities. The Suggested Reporting Language for the HIV Laboratory Diagnostic Testing Algorithm was updated in January 2019 and includes updates related to changes with the Geenius HIV-1/2 Supplemental Antibody Assay (FDA-approved 2017) as well as updates in the CDC Quick Reference Guide. The full recommendations still contain the most comprehensive information and are a useful resource for understanding the algorithm. 

HIV NAT Reference Centers

APHL manages two HIV Nucleic Acid Testing Reference Centers, New York State Department of Health Wadsworth Center and the Florida Bureau of Public Health Laboratories. The Reference Centers were set up in 2012 to ensure that HIV-1 NAT, the critical final step in the algorithm to reconcile discordant results, would be available to all public health laboratories. For labs that do not have an appropriate platform available, it is prohibitively expensive to bring NAT in-house. By virtue of the recommended algorithm, a single laboratory would have a relatively low volume of specimens requiring this technique. 

If you are interested in enrolling as a submitter to the HIV NAT Reference Centers, email sarah.buss@aphl.org

Integrating HIV and HCV Testing

APHL partnered with NASTAD to develop the Integrating HIV and HCV Testing Toolkit to assist public health programs and laboratories with identifying strategies to integrate testing at multiple levels. This toolkit and its associated slides will provide health departments (HDs) and laboratories with current information regarding HIV and HCV testing technologies, describe factors HDs should consider in determining when integration of testing is beneficial and discuss how various testing technologies and strategies for using these technologies can facilitate integration.