​​​​​​​​​​​​AP​​​​​​HL has developed numerous resources specific to STD laboratory practices. Additionally, we have compiled a number of external resources that could also be helpful in STD Testing. Check back frequently for updates and new resources. Non-APHL resources will indicate the primary source. ​

Issue Briefs, Factsheets and Considerations

Suggested Reporting Language for Syphilis Serology Testing, 2020

Mycoplasma genitalium: An Emerging Issue in the World of Sexually Transmitted Infections​, 2020​

Demystifying Multiple Neisseria gonorrhoeae Testing Methods and Results, 2019

FDA-approved or Cleared HIV, Viral Hepatitis​ and STD Assays by Manufacturer and Platform, 2021

The Role and Value of Public Health Laboratories in STD Response (Updated), 2017

Advances in Laboratory Detection of Trichomonas vaginalis (Updated), 2016

Recommendations and Best Practices

CDC: Sexually Transmitted Infections Treatment Guidelines, 2021

2017 Consultation on Laboratory Diagnosis of Syphilis-Final Meeting Report, 2018

Public Health Agency of Canada: Canadian Guideline​s on Sexually Transmitted Infections: Laboratory Diagnosis of Sexually Transmitte​​d Infections, 2018

CPHLN: ​Laboratory Guidelines for the Use of Serological Tests (Excluding Point of Care Tests) for the Diagnosis of Syphilis in Canada, 2015

​CDC: Recommendations for the Laboratory-Based Detection of Chlamydia trachomatis and Neisseria gonorrhoeae, 2014


STD Testing Practices in United States Public Health Laboratories:​ 2017 Survey Report, 2020, and Journal Article, 2019

Mycoplasma genitalium Laboratory Testing Survey Report, 2020

Chlamydia and Gonorrhea Clinical Laboratory Practices Survey: Survey Report 2013 and 2015, Summary Data Report and Data Dashboards, 2013

CDC: Sexually Transmitted Infections Surveillance, 2022

Laboratory Training Resources

CDC: Provider Performed Microscopy Procedures: A Focus on Quality Practices, 2021

Syphilis Serology Training Modules, 2020

How to Perform the ETEST Procedure: AST for Neisseria gonorrhoeae, 2020

CDC Laboratory Testing Resources  

CDC: Submitting Specimens to CDC for LGV Confirmation

CDC: Gonorrhea Laboratory Information

CDC: Gonococcal Isolate Surveillance Project

CDC: Neisseria gonorrhoeae Antimicrobial Resistance Susceptibility Testing

CDC: Spirochete Request Form

CDC: Syphilis Serum Repository

CDC: How to Request Laboratory Testing For STD (Searchable directory houses all CDC test orders, including Chlamydia, GUD, Neisseria, Syphilis Serology and Molecular) ​