​​​​​​​​​​​​AP​​​​​​HL has developed numerous resources specific to STD laboratory practices. Additionally, we have compiled a number of external resources that could also be helpful in STD Testing. Check back frequently for updates and new resources. Non-APHL resources will indicate the primary source. ​

Issue Briefs, Factsheets and Considerations

Suggested Reporting Language for Syphilis Serology Testing, 2020

​Mycoplasma genitalium: An Emerging Issue in the World of Sexually Transmitted Infections​, 2020​

Demystifying Multiple Neisseria gonorrhoeae Testing Methods and Results, 2019

FDA-approved or Cleared HIV, Viral Hepatitis​ and STD Assays by Manufacturer and Platform, 2018

The Role and Value of Public Health Laboratories in STD Response (Updated), 2017

CDC: Combating the Threat of Antibiotic-Resistant Gonorrhea, 2017

Advances in Laboratory Detection of Trichomonas vaginalis (Updated), 2016

Recommendations and Best Practices

CDC: Sexually Transmitted Infections Treatment Guidelines, 2021

2017 Consultation on Laboratory Diagnosis of Syphilis-Final Meeting Report, 2018

Public Health Agency of Canada: Canadian Guideline​s on Sexually Transmitted Infections: Laboratory Diagnosis of Sexually Transmitte​​d Infections, 2018

CPHLN: ​Laboratory Guidelines for the Use of Serological Tests (Excluding Point of Care Tests) for the Diagnosis of Syphilis in Canada, 2015

Transportation of Specimens for Neisseria gonorrhoeae Culture, 2014

CDC: Recommendations for the Laboratory-Based Detection of Chlamydia trachomatis and Neisseria gonorrhoeae, 2014


STD Testing Practices in United States Public Health Laboratories:​ 2017 Survey Report, 2020, and Journal Article, 2019

Mycoplasma genitalium Laboratory Testing Survey Report, 2020

Chlamydia and Gonorrhea Clinical Laboratory Practices Survey: Survey Report 2013 and 2015, Summary Data Report and Data Dashboards, 2013

CDC: Sexually Transmitted Diseases Surveillance, 2019

STD Testing Practices in United States Public Health Laboratories (Poster), 2012 

CDC: Volume and Type of Laboratory Testing Methods for Sexually Transmitted Diseases in Public Health Laboratories (2007), 2011

Laboratory Training Resources

CDC: Provider Performed Microscopy Procedures: A Focus on Quality Practices, 2016

Syphilis Serology Training Modules, 2020

How to Perform the ETEST Procedure: AST for Neisseria gonorrhoeae, 2020

CDC Laboratory Testing Resources  

CDC: Submitting Specimens to CDC for LGV Confirmation

CDC: Gonorrhea Laboratory Information

CDC: Gonococcal Isolate Surveillance Project

CDC: Neisseria gonorrhoeae Antimicrobial Resistance Susceptibility Testing

CDC: Spirochete Request Form

CDC: Syphilis Serum Repository

CDC: How to Request Laboratory Testing For STD (Specific Test Orders for Chlamydia, GUD, Neisseria, Syphilis Serology and Molecular) ​