​​Essentials for the Mycobacteriology Laboratory: Promoting Quality Practices

This series of free, interactive modules, developed by APHL in collaboration with the Centers for Disease Control and Prevention, promotes competency, quality and safety in common mycobacteriology testing practices. Designed for laboratorians at all levels of experience, each module in the series is complemented by references and resources. Modules are available in PDF format for use in laboratory training programs.

Users are encouraged to view the modules as frequently as needed. Additional modules are now in development. Please check this site frequently for new content.

*Note: Some of the Interactive Modules may not work in Chrome, please use Internet Explorer to open them.

Training Modules:

Overview of Tuberculosis

Laboratory Safety: Work Practices for Mycobacterium tuberculosis

Specimen Collection, Transport, Handling, and Processing

AFB Smear Microscopy

Overview of Mycobacterial Culture, Identification, and Drug Susceptibility Testing

Mycobacterial Culture

Identification of Mycobacteria

Drug Susceptibility Testing for MTB Complex

Molecular Biology 101

Molecular Detection and Identification of Mycobacteria

Molecular Detection of Drug Resistance

Landscape and Language of Molecular Diagnostics for TB Drug Resistance

Mycobacteriology False-Positive Case Studies **NEW MODULE**


In this module, laboratorians are provided an opportunity to navigate through five real-world cases in which a false-positive Mycobacterium tuberculosis result may have been reported. The scenarios include examples of pre-analytical, analytical and post-analytical processes. Participants will use this interactive module to help identify and investigate potential false-positive results.

Additional Resources

CDC False-Positive Toolkit


APHL sincerely thanks all who contributed their time and subject matter expertise to develop and produce these courses.

Project Leaders

Content Development

This project was supported by Cooperative Agreement # U60HM000803 from CDC and/or Assistant Secretary for Preparedness and Response. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of CDC and/or Assistant Secretary for Preparedness and Response.