The WGS Reference Centers will serve as an extension of the CDC DTBE Laboratory Branch and will provide services that are complementary to those at CDC. The centers will conduct one of the following approaches.

Option 1. Sequence samples provided by DTBE to support DTBE selected cluster investigations.

Option 2. Prospectively, a universal WGS surveillance pilot for a defined jurisdiction (e.g., city, county, region, state or multi-state).

Option 3. Provide a combination of both functions.

Proposals will be evaluated based on the laboratory’s existing capability and facilities and applicants must have:

  • Established capacity for preparing cultures of MTBC,
  • Established capacity for isolating chromosomal DNA from MTBC suitable for WGS,
  • Established and demonstrated capacity for WGS of bacterial pathogens and
  • Sufficient equipment, laboratory space and workforce capacity for the proposed testing volume


October 28, 2015 – RFP issued
November 5, 2015 – Informational Teleconference for RFP Q&A (see RFP for dial-in)
December 3, 2015  – RFP responses due
December 10, 2015 – Proposal review completed
December 10-17, 2015 – If needed, follow-up interviews and updated proposals due
December 17, 2015  – Final review completed and WGS Reference Center selected
January 15, 2016  – Contracts finalized and work begins

Request for Proposal Materials

The Establishment of Mycobacterium tuberculosis complex (MTBC) Whole Genome Sequencing Reference Centers RFP (updated 11.06.15) provides details on how to complete this RFP. The RFP contains a copy of the scoring criteria that will be used for evaluation of proposals in Appendix C. The example DNA extraction protocol provided by the CDC is now available (also included in the updated RFP above).

Questions & Answers

(click on headers below)

Does the page limit on the application include the budget?

No, the budget can be included as an appendix.

+ Eligibility

+ Award

+ Technical/Procedural Questions

+ Budgetary Questions

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​For more information on APHL's RFP process, visit the main RFP page.